Efficacy of Truffle on Serum Lipids and Antioxidant Status in Male Rats

Ibrahim Mahmoud1, Heba; Hamdy Haggag1, Mohammed; El-Sayed Mostafa2, Osama

doi:10.21608/enj.2025.432400

Efficacy of Truffle on Serum Lipids and Antioxidant Status in Male Rats

Document Type : Original Article

Authors

¹ 1- Nutrition and Food Sciences Department., Faculty of Home Economics, Helwan University, Cairo Egypt

² 2- Home economics Fa culty of Specific Education, Ain Shams University, Cairo, Egypt.

10.21608/enj.2025.432400

Abstract

ABSTRACT
This research was conducted in order to examine the efficacy of truffle on eliminating oxidative stress of male rats. Thirty-six male rats were divided into six groups (6 rats of each) as follow; The 1^stgroup was fed on basal diet as a negative control, the 2^ndgroup was as the same of group 1 and was intraperitoneally injected with potassium dichromate at a dose 10mg/kg BW as a positive control, the 3^rd, 4^th and 5^th as the same of group 2 with 300, 400 and 500 mg hydroethanolic truffle dried extract/Kg BW. Whereas, the 6^th group as the same of group 2 with 500 mg truffle powder /Kg BW, respectively. At the end of experiment (after 8 weeks), rats were fasted over night before scarifying. Serum kidney and liver functions as well as lipid profile, oxidative and anti-oxidative markers were analysed. Kidney and liver were removed from each rat to histopathological examination. The results indicated that truffle extract or its powder caused significant decreased (P˂0.05) in serum TC, TG, LDL-c, VLDL-c and MDA but significant increase in HDL-c and CAT compared to the +ve control group. Otherwise, histopathological examination showed improvement in kidney and liver of groups treated with truffle extract(showed improvement on kidney and liver of groups treated with truffle extract( as concentration 500mg group 5) or its powder(as concentration 500mg group 6) compared with +ve control group. According to these above results, the usage of truffle extract or its powder as natural agent may be effective in reducing the risk of oxidative stress so may play a therapeutic role of human beings.

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