Therapeutic evaluation of the anti-inflammatory and anti-oxidative protective effects of the ketogenic diet on Wister rats.

Background and Aim: Recent weight loss trends have favored the ketogenic low-carb diet. Due to conflicting studies, the carbohydrate restriction needed to induce ketosis is unknown. Ketogenic low-carbohydrate diets reduce weight and improve triglyceride and high-density lipoprotein levels better than low-fat diets. So, this study’s purpose is to examine the anti-inflammatory and anti-oxidative protective effects of a ketogenic diet on Wister rats’ liver by measuring different parameters.
Study Design: This study separated animals into four groups. The control group (GI) ate a usual diet of 24% protein, 58% carbs, and 18% fat for 10 weeks. The second group (GII) had a four-week high-fat diet (HFD). The third and fourth groups (GIII and GIV) followed the ketogenic diet (KD) for four and six weeks, respectively. After dissection, liver weights were assessed, homogenates were produced, oxidative stress biomarkers, liver function tests, and pro-inflammatory cytokines were analyzed using an enzyme-linked immunosorbent assay (ELISA).
Result: There was a reduction in liver weight observed in the animals following the administration of KD. Additionally, a decrease in liver enzyme levels was observed in both Groups GIII and GIV. Moreover, there is a significant disparity in oxidative stress between the corresponding group and the GI and GII groups. Furthermore, the proinflammatory cytokines levels (IL-6 and TNF-α) exhibited a decrease. It has been scientifically proven that the ketogenic diet possesses anti-inflammatory advantages.
Conclusion: The hepatoprotective effects of the ketogenic diet were demonstrated by reduced fat accumulation, anti-inflammatory effects, increased antioxidant defense, and weight loss potential in Wister Rats.